Gem-1 encodes an SLC16 monocarboxylate transporter-related protein that functions in parallel to the gon-2 TRPM channel during gonad development in Caenorhabditis elegans

Genetics. 2009 Feb;181(2):581-91. doi: 10.1534/genetics.108.094870. Epub 2008 Dec 15.

Abstract

The gon-2 gene of Caenorhabditis elegans encodes a TRPM cation channel required for gonadal cell divisions. In this article, we demonstrate that the gonadogenesis defects of gon-2 loss-of-function mutants (including a null allele) can be suppressed by gain-of-function mutations in the gem-1 (gon-2 extragenic modifier) locus. gem-1 encodes a multipass transmembrane protein that is similar to SLC16 family monocarboxylate transporters. Inactivation of gem-1 enhances the gonadogenesis defects of gon-2 hypomorphic mutations, suggesting that these two genes probably act in parallel to promote gonadal cell divisions. GEM-1GFP is expressed within the gonadal precursor cells and localizes to the plasma membrane. Therefore, we propose that GEM-1 acts in parallel to the GON-2 channel to promote cation uptake within the developing gonad.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / growth & development
  • Caenorhabditis elegans / metabolism*
  • Caenorhabditis elegans Proteins / chemistry
  • Caenorhabditis elegans Proteins / genetics*
  • Caenorhabditis elegans Proteins / metabolism*
  • Calcium / metabolism
  • Chromosome Mapping
  • DNA Primers / genetics
  • DNA, Helminth / genetics
  • Genes, Helminth*
  • Genes, Suppressor
  • Gonads / growth & development*
  • Gonads / metabolism*
  • Ion Channels / genetics*
  • Ion Channels / metabolism*
  • Models, Biological
  • Molecular Sequence Data
  • Monocarboxylic Acid Transporters / chemistry
  • Monocarboxylic Acid Transporters / genetics*
  • Monocarboxylic Acid Transporters / metabolism*
  • Mutation
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid

Substances

  • Caenorhabditis elegans Proteins
  • DNA Primers
  • DNA, Helminth
  • GON-2 protein, C elegans
  • Gem-1 protein, C elegans
  • Ion Channels
  • Monocarboxylic Acid Transporters
  • Recombinant Fusion Proteins
  • Calcium