Feasibility of ultrasound-guided percutaneous samplings in the pregnant baboon: a model for studies on transplacental transport

Reprod Sci. 2009 Mar;16(3):280-5. doi: 10.1177/1933719108324890. Epub 2008 Dec 15.


Objective: A study was conducted to test the feasibility of cordocenteses and amniocenteses at different gestational ages in pregnant baboons.

Study design: Experiments were performed in 10 pregnant baboons at a median gestational age of 131 (range 92-169) days. At different time intervals, percutaneous samplings of amniotic fluid and fetal blood were performed under ultrasound guidance. Simultaneously, maternal blood samples were drawn.

Results: With a median fetal weight of 431 g (range 111-690 g), 29 of 30 cordocenteses (96.6%) and all 30 amniocenteses and maternal samplings (100%) were successful in obtaining the required quantities for analysis. One cordocentesis was abandoned because of insufficient visualization of the umbilical cord due to a placental haemorrhage.

Conclusion: Percutaneous amniocentesis and cordocentesis can be performed with a high success rate in the pregnant baboon model. In combination with a 100% success rate in obtaining simultaneous maternal blood samples, this method is able to provide data on transplacental transport.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amniocentesis / methods*
  • Amniotic Fluid / metabolism
  • Animals
  • Antineoplastic Agents / blood
  • Antineoplastic Agents / pharmacokinetics
  • Birth Weight
  • Cordocentesis / methods*
  • Feasibility Studies
  • Female
  • Fetal Blood / metabolism
  • Gestational Age
  • Maternal-Fetal Exchange*
  • Models, Animal
  • Papio anubis
  • Placenta / blood supply*
  • Pregnancy
  • Ultrasonography, Interventional*
  • Umbilical Cord / diagnostic imaging*


  • Antineoplastic Agents