In vitro reduction of mutans streptococci by means of ozone gas application

Quintessence Int. 2008 Nov;39(10):827-31.


Objective: To evaluate, in vitro, the antimicrobial effect of ozone gas on strains of reference mutans streptococci and strains isolated from children at a high risk of developing caries.

Method and materials: A series of dilutions was obtained from a total of 41 strains, and the effect of ozone was determined for applications of 10, 20, and 40 seconds, as well as a control, in terms of the reduction of viable bacteria.

Results: Ozone application for 10 and 20 seconds produced a significant reduction in the number of bacteria, inversely proportional to the bacterial concentration. When exposure lasted 40 seconds, no viable bacteria were obtained.

Conclusions: Both time of application and initial bacterial concentration bear an influence on the antimicrobial effect of ozone on mutans streptococci.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child
  • Dental Disinfectants / pharmacology*
  • Humans
  • Ozone / pharmacology*
  • Streptococcus mutans / drug effects*


  • Dental Disinfectants
  • Ozone