Nanoparticle delivery of anti-metastatic NM23-H1 gene improves chemotherapy in a mouse tumor model

Cancer Gene Ther. 2009 May;16(5):423-9. doi: 10.1038/cgt.2008.97. Epub 2008 Dec 19.


Gene therapy provides a promising approach for cancer treatment. Earlier studies suggested that poly-L-lysine-modified iron oxide nanoparticles (IONP-PLL) might be a promising gene delivery system that can transfect DNA efficiently in vitro and in vivo. In this study we used IONP-PLL as gene carriers to deliver the NM23-H1 gene, the first suppressor gene of cancer metastasis, to tumor cells in vivo. The intravenous injection of IONP-PLL carrying NM23-H1-GFP plasmid DNA significantly extended the survival time of an experimental pulmonary metastasis mouse model. In the IONP-PLL/NM23-H1-GFP-treated group, metastasis was clearly suppressed compared with the group treated with free NM23-H1-GFP plasmid. Furthermore, this gene therapy combined with cyclophosphamide treatment resulted in longer survival times and greater suppression of metastasis growth. In conclusion, treatment with IONP-PLL nanoparticles incorporating the NM23-H1gene is an efficient gene therapy method, and it is even more effective in combination with chemotherapy. This approach appears to be a promising strategy for treatment of metastatic tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology*
  • Antineoplastic Agents / therapeutic use
  • Cell Line, Tumor
  • Disease Models, Animal
  • Gene Expression Regulation, Neoplastic
  • Gene Transfer Techniques*
  • Genetic Therapy*
  • Genetic Vectors* / administration & dosage
  • Genetic Vectors* / toxicity
  • Inhibitor of Apoptosis Proteins
  • Mice
  • Mice, Inbred C57BL
  • NM23 Nucleoside Diphosphate Kinases / administration & dosage*
  • NM23 Nucleoside Diphosphate Kinases / metabolism
  • Nanoparticles / therapeutic use*
  • Neoplasm Metastasis
  • Survivin
  • Zebrafish Proteins


  • Antineoplastic Agents
  • Birc5a protein, zebrafish
  • Inhibitor of Apoptosis Proteins
  • NM23 Nucleoside Diphosphate Kinases
  • Survivin
  • Zebrafish Proteins
  • NME1 protein, human