Identification and functional analysis of brassicicene C biosynthetic gene cluster in Alternaria brassicicola

Bioorg Med Chem Lett. 2009 Feb 1;19(3):870-4. doi: 10.1016/j.bmcl.2008.11.108. Epub 2008 Dec 6.

Abstract

The biosynthetic gene cluster of brassicicene C was identified in Alternaria brassicicola strain ATCC 96836 from genome database search. In vivo and in vitro study clearly revealed the function of Orf8 and Orf6 as a fusicoccadiene synthase and methyltransferase, respectively. The understanding toward the biosynthetic pathway promises construction of this type of diterpene compounds with genetic engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternaria / genetics*
  • Cell-Free System
  • Chemistry, Pharmaceutical / methods
  • Chromatography, Gas / methods
  • Chromatography, High Pressure Liquid
  • Culture Media
  • Diterpenes / chemistry*
  • Drug Design
  • Electrophoresis, Polyacrylamide Gel
  • Genes, Bacterial*
  • Genome
  • Methyltransferases / genetics
  • Models, Chemical
  • Models, Genetic
  • Multigene Family*
  • Plant Extracts / metabolism*

Substances

  • Culture Media
  • Diterpenes
  • Plant Extracts
  • brassicicene C
  • Methyltransferases