Disappearance of Nine Monoterpenes Exposed in Vitro to the Rumen Microflora of Dairy Goats: Effects of Inoculum Source, Redox Potential, and Vancomycin

J Anim Sci. 2009 Apr;87(4):1366-73. doi: 10.2527/jas.2008-1419. Epub 2008 Dec 19.

Abstract

This in vitro study evaluated the roles of diet type and redox potential in the degradation of linalool, (E)- and (Z)-beta-ocimene, alpha-phellandrene, (-)-beta-pinene, (-)-alpha-pinene, (+)-alpha-pinene, sabinene, and alpha-terpinene when incubated with rumen microflora, and it provided information on the time course of their disappearance. The 9 monoterpenes are found in the winter and spring diets of dairy goats in northwestern Mediterranean grazing systems. The diets were individually exposed to rumen microflora for 3 h in 17-mL culture tubes at a concentration of 4 microL/L. The mixed flora of the inoculum was controlled by the use of vancomycin (eliminating gram-positive bacteria) and by the energy source (starch vs. structural polysaccharides) on which rumen microflora had been grown. Redox potential was controlled by addition of L-cysteine-hydrochloride. The preliminary adaptation of microbial inoculum to a diet rich in structural carbohydrates reduced the recovery yields of (E)- and (Z)-beta-ocimene, (-)-beta-pinene, (-)-alpha-pinene, (+)-alpha-pinene, and sabinene (P<0.01), whereas vancomycin was without effect. The effect of carbohydrate source likely stems from the specific composition of the microbial community rather than from its acidogenic capacity. Reducing the culture redox potential by 50 mV reduced the recovery yields of linear and monocyclic terpenes (P<0.02), and the culture redox potential interacted with the inoculum source for (E)- and (Z)-beta-ocimene and for alpha-phellandrene. The time course of terpene disappearance was studied by exposing terpenes to a rumen microflora adapted either to starch or to fiber for 3, 6, or 24 h. The degraded fraction reached a plateau within 3 h for alpha-phellandrene and for all the isomers of beta-ocimene and pinene with the fiber-adapted microflora as well as with both inocula for alpha-terpinene. With the starch-adapted microflora, this steady state was reached for most other terpenes within 6 h of incubation. Sabinene was the only compound still disappearing after an incubation period of 6 h. Biotic and environmental variables in the rumen affected terpene degradation in a way that can alter the dietary terpene profile and possibly its influence on animal product characteristics.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Dairying
  • Goats / metabolism*
  • Male
  • Monoterpenes / pharmacokinetics*
  • Oxidation-Reduction*
  • Rumen* / drug effects
  • Rumen* / metabolism
  • Rumen* / microbiology
  • Time Factors
  • Vancomycin / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Monoterpenes
  • Vancomycin