Assaying beta-catenin/TCF transcription with beta-catenin/TCF transcription-based reporter constructs

Methods Mol Biol. 2008;468:99-110. doi: 10.1007/978-1-59745-249-6_8.

Abstract

Transcription-based reporters have been instrumental in characterizing the Wnt/beta-catenin signaling pathway and will be essential in the search for therapeutics aimed at combating diseases linked to aberrant signaling. In this chapter, we introduce a new improved Wnt/beta-catenin reporter system, beta-catenin-activated reporter (BAR), and its accompanying control reporter system, found unresponsive BAR (fuBAR). Its enhanced sensitivity, increased dynamic range, and lentiviral platform provide a reporter system that will keep pace with the needs of scientists in the field.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Assay / methods*
  • Cell Line
  • Genes, Reporter*
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • Humans
  • Lentivirus / genetics
  • Lentivirus / metabolism
  • Signal Transduction / physiology
  • TCF Transcription Factors / genetics
  • TCF Transcription Factors / metabolism*
  • Transcription, Genetic*
  • beta Catenin / genetics
  • beta Catenin / metabolism*

Substances

  • TCF Transcription Factors
  • beta Catenin