Objectives: To measure serum cholinesterase (SCHE) with an integration strategy.
Design and methods: At 54.0 micromol/L butyrylthiolcholine, SCHE initial rates were calculated with 50.0 micromol/L butyrylthiolcholine and maximal rates via an improved integrated method if substrate consumptions within 5.0 min were over 60%, or were determined by the classical initial rate method.
Results: The linear range was from 16 to 1560 nkat/L, and SCHE in clinic sera showed negligible substrate-activation.
Conclusion: This strategy was effective.