A novel, anaerobically induced ferredoxin in Chlamydomonas reinhardtii

FEBS Lett. 2009 Jan 22;583(2):325-9. doi: 10.1016/j.febslet.2008.12.018. Epub 2008 Dec 26.


We have found the transcript of one of at least six ferredoxin encoding genes of the green alga Chlamydomonas reinhardtii, FDX5, strongly accumulating in anaerobiosis, indicating a vital role of the encoded protein in the anaerobic metabolism of the cells. According to absorption and electron paramagnetic resonance spectroscopy, Fdx5 is a plant-type [2Fe2S]-ferredoxin with a redox potential similar to that of the ferredoxin PetF. However, although Fdx5 seems to be located in the chloroplast, it is not able to photoreduce nicotinamide adenine dinucleotide phosphate (NADP(+)) via ferredoxin-NADP-reductase, nor to be an electron donor to the plastidic [FeFe]-hydrogenase HydA1. Thus, Fdx5 seems to have a special role in a yet to be identified anaerobic pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaerobiosis
  • Animals
  • Chlamydomonas reinhardtii / enzymology*
  • Chlamydomonas reinhardtii / genetics
  • Ferredoxin-NADP Reductase / metabolism
  • Ferredoxins / genetics
  • Ferredoxins / metabolism*
  • Oxidation-Reduction
  • Oxidoreductases / metabolism
  • RNA, Messenger / biosynthesis
  • Transcription, Genetic


  • 2Fe-2S ferredoxin
  • Ferredoxins
  • RNA, Messenger
  • Oxidoreductases
  • uptake hydrogenase
  • Ferredoxin-NADP Reductase