Mutations of RAS gene family in specimens of bladder cancer

Urol J. Fall 2008;5(4):237-42.

Abstract

Introduction: Studies have shown different types of RAS mutations in human bladder tumors with a wide range of mutation frequencies in different patient populations. This study aimed to assess the frequency of specific-point mutations in the RAS gene family of a group of Iranian patients with bladder cancer.

Materials and methods: We examined the tumor specimens of 35 consecutive patients with transitional cell carcinoma. The DNA samples were evaluated for the occurrence of HRAS, KRAS, and NRAS activation using a polymerase chain reaction-restriction fragment length polymorphism technique.

Results: None of the patients had mutations in the RAS gene family "hot spots" including codons 12, 13, and 61.

Conclusion: We failed to find RAS mutations in our bladder tumor samples. These observations may reflect the involvement of different etiological factors in the induction of bladder tumor of which RAS mutation might not be present in all populations.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma, Transitional Cell / genetics*
  • Carcinoma, Transitional Cell / metabolism
  • Carcinoma, Transitional Cell / pathology
  • Codon / genetics
  • Cohort Studies
  • Female
  • Genes, ras / genetics*
  • Humans
  • Iran
  • Male
  • Middle Aged
  • Point Mutation / genetics*
  • Polymerase Chain Reaction
  • Urinary Bladder Neoplasms / genetics*
  • Urinary Bladder Neoplasms / metabolism
  • Urinary Bladder Neoplasms / pathology
  • ras Proteins / metabolism

Substances

  • Codon
  • ras Proteins