Released Exopolysaccharide (r-EPS) Produced From Probiotic Bacteria Reduce Biofilm Formation of Enterohemorrhagic Escherichia Coli O157:H7

Biochem Biophys Res Commun. 2009 Feb 6;379(2):324-9. doi: 10.1016/j.bbrc.2008.12.053. Epub 2008 Dec 25.

Abstract

Here, we characterized released-exopolysaccharides (r-EPS) from Lactobacillus acidophilus A4 with the goal of identifying natural compounds that represses biofilm formation. In plastic 96-well microplates that contained 1.0 mg/ml of r-EPS, enterohemorrhagic Escherichia coli (EHEC) biofilms were dramatically decreased by 87% and 94% on polystyrene and polyvinyl chloride (PVC) surfaces, respectively. In the presence of r-EPS, neither their growth rate nor their autoinducer-2-like activity was affected on the EHEC O157:H7. Importantly, consistent reduction in biofilm formation was also observed when r-EPS was applied to the continuous-flow chamber models. In addition, we found that adding r-EPS significantly repressed biofilm formation by affecting genes related to curli production (crl, csgA, and csgB) and chemotaxis (cheY) in transcriptome analysis. Furthermore, these r-EPS could prevent biofilm formation by a wide range of Gram-negative and -positive pathogens. This property may lead to the development of novel food-grade adjuncts for microbial biofilm control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Biofilms / growth & development*
  • Cell Line, Tumor
  • Chemotaxis / genetics
  • Escherichia coli Infections / immunology*
  • Escherichia coli O157 / genetics
  • Escherichia coli O157 / immunology
  • Escherichia coli O157 / physiology*
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial
  • Humans
  • Lactobacillus acidophilus / immunology*
  • Polysaccharides, Bacterial / immunology*
  • Polystyrenes
  • Polyvinyl Chloride
  • Probiotics*

Substances

  • Bacterial Proteins
  • Polysaccharides, Bacterial
  • Polystyrenes
  • Crl protein, Bacteria
  • Polyvinyl Chloride