[Efficacy of activated lymphocytes transfer therapy as a novel maker for serum granulysin level with advanced gastric cancer patients]

Gan To Kagaku Ryoho. 2008 Nov;35(12):2262-4.
[Article in Japanese]


Introduction: Granulysin is a cytotoxic granule protein of cytotoxic T-lymphocytes and natural killer cells. Gastric cancer patients of stage II and stage III were reported to have pre-operative serum granulysin, which is a good prognostic factor. Activated T lymphocytes produce a lot of granulysin in the cell. We hypothesized that activated T lymphocytes transfer therapy may increase in serum granulysin leveles of stage IV and relapsed patients.

Method: Between April 2002 and December 2007, the patients received a standard therapy with activated lymphocytes transfer therapy. Peripheral blood samples were taken from the patients. Serum granulysin concentrations were measured using a granulysin-specific ELISA kit.

Result: The average serum granulysin level for Stage IV (n=29) and relapsed patients (n=13) was 3.3 ng/mL. The gastric cancer patients were classified into two groups based on their concentration of serum granulysin level: high group (> or = 3.3 ng/mL); low group (< 3.3 ng/mL). There were no significant differences between these two groups in gender and age. The stage IV high group had a longer survival time than the stage IV low group. After 60 days from the activated T lymphocytes transfer therapy, the average pre-treatment serum granulysin had increased. However, there was no significant difference to note. It is noteworthy that serum granulysin is a novel parameter of patients cancer immunity.

Publication types

  • English Abstract

MeSH terms

  • Antigens, Differentiation, T-Lymphocyte / blood*
  • Biomarkers / blood
  • Humans
  • Immunotherapy*
  • Liver Neoplasms / blood
  • Liver Neoplasms / immunology
  • Liver Neoplasms / secondary
  • Liver Neoplasms / therapy
  • Lymphocyte Activation / immunology*
  • Lymphocyte Transfusion*
  • Lymphocytes / immunology*
  • Neoplasm Staging
  • Stomach Neoplasms / blood
  • Stomach Neoplasms / immunology*
  • Stomach Neoplasms / pathology
  • Stomach Neoplasms / therapy*
  • Survival Rate


  • Antigens, Differentiation, T-Lymphocyte
  • Biomarkers
  • GNLY protein, human