Stimulation of osteoblastic differentiation and inhibition of interleukin-6 and nitric oxide in MC3T3-E1 cells by pomegranate ethanol extract

Phytother Res. 2009 May;23(5):737-9. doi: 10.1002/ptr.2587.


In this experiment, we studied the effects of pomegranate fruit extract (PE) on the function of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts. PE (16 approximately 250 microg/ml) significantly increased the growth of MC3T3-E1 cells (P < 0.05). Moreover, PE (50 microg/ml) caused a significant elevation of alkaline phosphatase (ALP) activity and collagen content in the cells. We then examined the effect of PE on the TNF-alpha-induced production of interleukin-6 (IL-6) and nitric oxide (NO) in osteoblasts. Treatment with PE (10 approximately 50 microg/ml) decreased the TNF-alpha (10(-10) M)-induced production of IL-6 and NO in osteoblasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Cell Differentiation / drug effects*
  • Cell Line
  • Collagen / metabolism
  • Interleukin-6 / metabolism*
  • Lythraceae / chemistry
  • Mice
  • Nitric Oxide / metabolism*
  • Osteoblasts / drug effects*
  • Plant Extracts / pharmacology*
  • Tumor Necrosis Factor-alpha / metabolism


  • Interleukin-6
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide
  • Collagen
  • Alkaline Phosphatase