An experiment was conducted to assess the effects of two-stage weaning and subsequent transport on oxidative stress markers in calves. Thirty-six crossbred Angus steers (243+/-20.8 kg; 221+/-19.7 days) were allotted to control, fenceline (FL), and nose-clip (NC) weaning groups. Steers were weighed and blood samples were collected on days -7, 0 (pre-transit) and 7 surrounding transport. Leukocytes were analyzed for glutathione peroxidase and glutathione reductase activity, whereas malondialdehyde and Se were measured in plasma. Data were analyzed using PROC MIXED with the Tukey-Kramer adjustment applied post hoc. No treatment effects were detected for blood variables or weights. Glutathione peroxidase activity was not altered by time (P = 0.27). Glutathione reductase activity, Se, and malondialdehyde declined (P<0.001) from day -7 to 7 in all steers. Treatmentxdate interaction was detected for malondialdehyde (P<0.02). Control and FL steers gained weight (P<0.03), whereas NC steers did not gain weight between days -7 and 0 (P = 0.81). All weaning treatment groups gained weight between days 0 and 7 (P<0.05). Results suggest that two-stage weaning has little effect on examined markers of oxidative stress.