Analysis of cytoplasmic mRNA decay in Saccharomyces cerevisiae

Methods Enzymol. 2008;448:409-27. doi: 10.1016/S0076-6879(08)02620-7.

Abstract

The yeast, Saccharomyces cerevisiae, is a model system for the study of eukaryotic mRNA degradation. In this organism, a variety of methods have been developed to measure mRNA decay rates, trap intermediates in the mRNA degradation process, and establish precursor-product relationships. In addition, the use of mutant strains lacking specific enzymes involved in mRNA destruction, or key regulatory proteins, allows one to determine the mechanisms by which individual mRNAs are degraded. In this chapter, we discuss methods for analyzing mRNA degradation in S. cerevisiae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytoplasm / genetics*
  • RNA Stability / genetics*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / genetics*

Substances

  • RNA, Messenger