Targeted disruption of the mouse Lipoma Preferred Partner gene

Biochem Biophys Res Commun. 2009 Feb 6;379(2):368-73. doi: 10.1016/j.bbrc.2008.12.074. Epub 2008 Dec 26.


LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance in heterozygous crosses suggested partial embryonic lethality of Lpp(-/-) females. Fertility of Lpp(-/-) males was proven to be normal, however, females from Lpp(-/-) x Lpp(-/-) crosses produced a strongly reduced number of offspring, probably due to a combination of female embryonic lethality and aberrant pregnancies. Apart from these developmental and reproductive abnormalities, Lpp(-/-) mice that were born reached adulthood without displaying any additional macroscopic defects. On the other hand, Lpp(-/-) mouse embryonic fibroblasts exhibited reduced migration capacity, reduced viability, and reduced expression of some Lpp interaction partners. Finally, we discovered a short nuclear form of Lpp, expressed mainly in testis via an alternative promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Nucleus / metabolism
  • Cytoskeletal Proteins / genetics
  • Cytoskeletal Proteins / metabolism
  • Cytoskeletal Proteins / physiology*
  • Embryo Loss / genetics
  • Embryonic Development / genetics*
  • Female
  • Fertility / genetics*
  • Fibroblasts / metabolism
  • Gene Targeting
  • LIM Domain Proteins
  • Male
  • Mice
  • Mice, Knockout
  • Promoter Regions, Genetic
  • Protein Biosynthesis / genetics
  • Reproduction / genetics
  • Testis / metabolism
  • Transcription, Genetic


  • Cytoskeletal Proteins
  • LIM Domain Proteins
  • Lpp protein, mouse