Embryonic expression of Drosophila IMP in the developing CNS and PNS

Gene Expr Patterns. 2009 Mar;9(3):138-43. doi: 10.1016/j.gep.2008.12.001. Epub 2008 Dec 11.


Drosophila IMP (dIMP) is related to the vertebrate RNA-binding proteins IMP1-3, ZBP1, Vg1RBP and CRD-BP, which are involved in RNA regulatory processes such as translational repression, localization and stabilization. The proteins are expressed in many fetal tissues, including the developing nervous system, and IMP up-regulation in solid tumors correlates with a high metastatic potential and poor prognosis. In this study, we used immunohistochemistry and live-imaging of an endogenous promoter-driven GFP-dIMP fusion protein to reveal the expression pattern of dIMP protein throughout embryogenesis. In the cellular blastoderm, immunoreactivity was seen in the entire cell-layer, where it was localized apically to the nucleus, and in the pole cells. Later, the GFP-dIMP fusion protein appeared in the developing central nervous system, both in the brain and in the ventral nerve cord. In the peripheral nervous system, immunoreactivity was detected in both neurons and accessory cells of chordotonal and external sensory organs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastoderm / metabolism
  • Brain / embryology
  • Brain / metabolism
  • Drosophila Proteins* / biosynthesis
  • Drosophila Proteins* / genetics
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Epidermis / embryology
  • Epidermis / metabolism
  • Gene Expression
  • Gene Expression Profiling
  • Green Fluorescent Proteins
  • Immunohistochemistry
  • Microscopy, Confocal
  • RNA-Binding Proteins* / biosynthesis
  • RNA-Binding Proteins* / genetics
  • Recombinant Fusion Proteins
  • Sensory Receptor Cells / metabolism


  • Drosophila Proteins
  • Imp protein, Drosophila
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins