Objective: To test different methods for extracting volatile oil from bergamot.
Methods: The determination of bergapten was carried out by RP-HPLC. Four different ways of organic solvent extraction, steam-input distillation, distillation of the material mixed with water and press extraction were compared.
Results: Bergapten wasnt extracted by ways of steam-input distillation and distillation of the material mixed with water.
Conclusion: The steam distillation extraction can be taken to extract volatile oil from bergamot for protecting humans' skins.