A novel dengue vaccine candidate that induces cross-neutralizing antibodies and memory immunity

Microbes Infect. 2009 Feb;11(2):288-95. doi: 10.1016/j.micinf.2008.12.004. Epub 2008 Dec 16.

Abstract

A novel dengue vaccine candidate comprised of a consensus dengue virus envelope protein domain III (cED III) was developed to fight against dengue virus infection. The amino acid sequence of this novel cED III was obtained by alignment of amino acid sequences from different isolates of the four serotypes of dengue viruses. A proof-of-concept study demonstrated that BALB/c mice immunized with the recombinant cED III developed neutralizing antibodies against all serotypes of dengue virus. Moreover, formulation of recombinant cED III with aluminum phosphate could induce long-lasting antibody responses and anamnestic neutralizing antibody responses following challenge with dengue virus at week 28 after priming. These results demonstrate the possibility of developing a single tetravalent vaccine against dengue viral infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / administration & dosage
  • Adjuvants, Immunologic / pharmacology
  • Aluminum Compounds / administration & dosage
  • Aluminum Compounds / pharmacology
  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral / blood*
  • Consensus Sequence
  • Dengue Vaccines / genetics
  • Dengue Vaccines / immunology*
  • Dengue Virus / genetics
  • Dengue Virus / immunology*
  • Immunologic Memory*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Neutralization Tests
  • Phosphates / administration & dosage
  • Phosphates / pharmacology
  • Sequence Alignment
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / immunology
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology

Substances

  • Adjuvants, Immunologic
  • Aluminum Compounds
  • Antibodies, Viral
  • Dengue Vaccines
  • E-glycoprotein, Dengue virus type 2
  • Phosphates
  • Vaccines, Synthetic
  • Viral Envelope Proteins
  • aluminum phosphate