In vitro cytotoxicity of a novel injectable and biodegradable alveolar bone substitute

Biochem Biophys Res Commun. 2009 Feb 6;379(2):557-61. doi: 10.1016/j.bbrc.2008.12.084. Epub 2008 Dec 29.


The unsaturated polyphosphoester (UPPE) polymer is being investigated as an injectable and biodegradable system for alveolar bone repair in the treatment of periodontal diseases. The incorporation of beta-tricalcium phosphate (beta-TCP) particles into the UPPE polymer was previously shown to significantly increase the material's mechanical properties. Moreover, in vitro experiments demonstrated that the UPPE/beta-TCP composite was capable of zero-order release of tetracycline for over 2 weeks. In this study, we investigated the in vitro cytotoxicity of each individual component, the resulting cross-linked network and the degradation products of the UPPE/beta-TCP composite using an AlamarBlue viability assay. We confirmed that each individual component except beta-TCP and the in vitro degradation products of the composite displayed a dose-dependent cytotoxic response. Once cross-linked, however, the composite did not demonstrate an adverse response. Our results suggest that the UPPE/beta-TCP composite holds great promise for use as an injectable and biodegradable alveolar bone substitute.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorbable Implants*
  • Alveolar Bone Loss / surgery
  • Animals
  • Bone Substitutes / administration & dosage
  • Bone Substitutes / chemistry
  • Bone Substitutes / toxicity*
  • Calcium Phosphates / administration & dosage
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / toxicity*
  • Cell Line
  • Cell Survival / drug effects
  • Fibroblasts / drug effects
  • Mice
  • Organophosphates / administration & dosage
  • Organophosphates / chemistry
  • Organophosphates / toxicity*
  • Polyesters / administration & dosage
  • Polyesters / chemistry
  • Polyesters / toxicity*


  • Bone Substitutes
  • Calcium Phosphates
  • Organophosphates
  • Polyesters
  • beta-tricalcium phosphate