Characterization of the Caenorhabditis elegans Islet LIM-homeodomain ortholog, lim-7

FEBS Lett. 2009 Jan 22;583(2):456-64. doi: 10.1016/j.febslet.2008.12.046. Epub 2008 Dec 29.


lim-7 is one of seven Caenorhabditis elegans LIM-homeodomain (LIM-HD)-encoding genes and the sole Islet ortholog. LIM-HD transcription factors, including Islets, function in neuronal and non-neuronal development across diverse phyla. Our results show that a lim-7 deletion allele causes early larval lethality with terminal phenotypes including uncoordination, detached pharynx, constipation and morphological defects. A lim-7(+) transgene rescues lethality but not adult sterility. A lim-7(+) reporter in the full genomic context is expressed in all gonadal sheath cells, URA neurons, and additional cells in the pharyngeal region. Finally, we identify a 45-bp regulatory element in the first intron that is necessary and sufficient for lim-7 gonadal sheath expression.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Caenorhabditis elegans / anatomy & histology
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / growth & development*
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • Gene Expression Regulation, Developmental
  • Genes, Essential
  • Genes, Lethal
  • Gonads / anatomy & histology
  • Gonads / growth & development
  • Gonads / metabolism
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism*
  • LIM-Homeodomain Proteins
  • Molecular Sequence Data
  • Phenotype
  • RNA Interference
  • Regulatory Elements, Transcriptional


  • Caenorhabditis elegans Proteins
  • Homeodomain Proteins
  • LIM-Homeodomain Proteins
  • Lim-7 protein, C elegans