Use of pradofloxacin to treat experimentally induced Mycoplasma hemofelis infection in cats

Am J Vet Res. 2009 Jan;70(1):105-11. doi: 10.2460/ajvr.70.1.105.

Abstract

Objective: To evaluate the efficacy of the fluoroquinolone pradofloxacin in the treatment of cats experimentally infected with Mycoplasma hemofelis.

Animals: 23 young adult specific-pathogen-free cats.

Procedures: Cats were inoculated with M hemofelis from a chronically infected donor and assigned to 1 of 4 treatment groups: a doxycycline group, a low-dose-pradofloxacin group, a high-dose-pradofloxacin group, and an untreated control group. Treatment was initiated for 14 days when M hemofelis infection was detected via PCR assay and clinical signs of hemoplasmosis were present. Cats that had negative PCR assay results after treatment were administered a glucocorticoid and monitored via PCR assay for an additional 4 weeks.

Results: All cats yielded positive results for M hemofelis via conventional PCR and quantitative PCR assays and developed anemia. The low-dose-pradofloxacin group had significantly lower M hemofelis copy numbers than the doxycycline group. Six cats treated with pradofloxacin yielded negative results during treatment. Of those cats, 4 yielded negative conventional PCR assay results and all yielded negative quantitative PCR assay results for M hemofelis 1 month after administration of high-dose glucocorticoids.

Conclusions and clinical relevance: Pradofloxacin had anti-M hemofelis effects similar to those of doxycycline. In addition, pradofloxacin may be more effective at long-term M hemofelis organism clearance than doxycycline.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anemia / drug therapy
  • Anemia / metabolism
  • Anemia / microbiology
  • Anemia / veterinary
  • Animals
  • Anti-Bacterial Agents / pharmacokinetics
  • Anti-Bacterial Agents / pharmacology*
  • Blood Cell Count / veterinary
  • Cat Diseases / drug therapy*
  • Cat Diseases / metabolism
  • Cat Diseases / microbiology
  • Cats
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Doxycycline / pharmacokinetics
  • Doxycycline / pharmacology
  • Female
  • Fluoroquinolones / pharmacokinetics
  • Fluoroquinolones / pharmacology*
  • Hematocrit / veterinary
  • Male
  • Mycoplasma / genetics
  • Mycoplasma / growth & development*
  • Mycoplasma Infections / drug therapy
  • Mycoplasma Infections / metabolism
  • Mycoplasma Infections / microbiology
  • Mycoplasma Infections / veterinary*
  • Polymerase Chain Reaction / veterinary
  • Random Allocation
  • Specific Pathogen-Free Organisms

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Fluoroquinolones
  • pradofloxacin
  • Doxycycline