Tumor necrosis factor alpha inducing spatial interactions between calcium-sensing receptor and L-type voltage-dependent calcium channel

Ann N Y Acad Sci. 2008 Dec;1150:320-2. doi: 10.1196/annals.1447.030.


The temporal and spatial regulation of intracellular calcium concentration ([Ca(2+)](i)) is very crucial for maintaining calcium ion homeostasis within cells, and consequently in the regulation of cellular functions such as beta cell replication and differentiation, insulin secretion, and apoptosis. Calcium ion regulatory proteins playing major roles in these processes include L-type voltage-dependent calcium channels (L-type VDCCs) and calcium-sensing receptors (CaRs). Tumor necrosis factor alpha (TNF-alpha), a cytokine, is widely known to activate nuclear factor-kappa B (NF-kappaB) transcription in beta cells. Confocal fluorescence imaging data suggest increased co-localization of CaRs with L-type VDCCs upon treatment of beta cells with TNF-alpha, thereby indicating increased membrane-delimited spatial interactions between these two membrane proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium Channels, L-Type / metabolism*
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Insulin-Secreting Cells / drug effects
  • Insulin-Secreting Cells / metabolism
  • Protein Binding / drug effects
  • Rats
  • Receptors, Calcium-Sensing / metabolism*
  • Tumor Necrosis Factor-alpha / pharmacology*


  • Calcium Channels, L-Type
  • Receptors, Calcium-Sensing
  • Tumor Necrosis Factor-alpha