Cholestane-3beta,5alpha,6beta-triol-induced reactive oxygen species production promotes mitochondrial dysfunction in isolated mice liver mitochondria

Chem Biol Interact. 2009 May 15;179(2-3):81-7. doi: 10.1016/j.cbi.2008.12.003. Epub 2008 Dec 11.

Abstract

Reactive oxygen species (ROS) have been implicated in oxysterol-induced apoptosis. However, the mechanism of ROS production induced by oxysterols within cells is not clear. Considering that mitochondria is the main source of intracellular ROS, and play a key role in oxysterol-induced apoptosis, we investigated the effect of oxysterol cholestane-3beta,5alpha,6beta-triol (Triol) on ROS production and mitochondrial function in isolated mice liver mitochondria. Triol at higher concentrations (10-50 microM) enhanced the production of O(2)(-) and H(2)O(2) in isolated mitochondria, which might be due to its stimulation to the activities of complexes I and II of mitochondrial electron transfer chain, and its inhibition to glutathione peroxidase activity. The same concentrations of Triol induced obviously oxidative damage of mitochondrial membrane lipids and proteins, as demonstrated by the increased MDA level and the decreased protein thiols content. Furthermore, Triol caused mitochondrial dysfunction, including the opening of mitochondrial permeability transition pore, the decrease of mitochondrial membrane potential (DeltaPsi(m)), and the release of cytochrome c. Antioxidant butylated hydroxytoluene significantly inhibited oxidative damage, the decrease of DeltaPsi(m), and the release of cytochrome c, implying that ROS might mediate mitochondrial dysfunction induced by Triol. We concluded that Triol-induced mitochondrial ROS production and subsequently oxidative damage, leading to the mitochondrial dysfunction, thus suggesting a putative mechanism of apoptosis activation by oxysterols in vascular cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Cholestanols / pharmacology*
  • Cholesterol / analogs & derivatives
  • Cholesterol / pharmacology
  • Cytochromes c / drug effects
  • Cytochromes c / metabolism
  • Dose-Response Relationship, Drug
  • Electron Transport / drug effects
  • Glutathione Peroxidase / antagonists & inhibitors
  • Glutathione Peroxidase / metabolism
  • Hydrogen Peroxide / metabolism
  • Lipid Peroxidation / drug effects
  • Mice
  • Mitochondria, Liver / drug effects*
  • Mitochondria, Liver / enzymology
  • Mitochondria, Liver / metabolism
  • Mitochondria, Liver / pathology*
  • Oxygen / metabolism
  • Reactive Oxygen Species / metabolism*
  • Sulfhydryl Compounds / metabolism
  • Superoxide Dismutase / antagonists & inhibitors
  • Superoxide Dismutase / metabolism

Substances

  • Cholestanols
  • Reactive Oxygen Species
  • Sulfhydryl Compounds
  • cholestane-3,5,6-triol
  • Cytochromes c
  • Cholesterol
  • Hydrogen Peroxide
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Oxygen