Current application and technology of functional multineuron calcium imaging

Biol Pharm Bull. 2009 Jan;32(1):1-9. doi: 10.1248/bpb.32.1.


Here we describe recent applications and technical advancements of functional multineuron calcium imaging (fMCI), which monitors the firing activity of more than a thousand neurons through their somatic Ca(2+) signals. fMCI is used for analysis of various neural circuits under normal and pathological conditions. In vitro fMCI is made more sophisticated by using multipoint illumination and scanning technology with spinning-disk and low-laser-intensity imaging, electron-multiplying charge-coupled device cameras, etc. In vivo fMCI is still developing. We review optical technologies for fast scanning imaging, deep tissue imaging, and recording from moving animals.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Action Potentials / physiology
  • Animals
  • Calcium / metabolism*
  • Calcium Signaling / physiology*
  • Diagnostic Imaging / instrumentation
  • Diagnostic Imaging / methods*
  • Humans
  • Neurons / metabolism*


  • Calcium