Anti-hepatitis B virus activities of astragaloside IV isolated from radix Astragali

Biol Pharm Bull. 2009 Jan;32(1):132-5. doi: 10.1248/bpb.32.132.


Total ethanol extract and saponins from Chinese herb radix Astragali (huangqi) have been previously shown to possess anti-hepatitis B virus (HBV) activities in vitro. To identify the active ingredients, we isolated a triterpenoid saponin that was determined to be astragaloside IV. In the human HBV-transfected liver cell line HepG(2) 2.2.15, astragaloside IV effectively suppressed secretion of HBV antigens with inhibition rates of 23.6% for the secretion of Hepatitis B surface antigen (HBsAg) and 22.9% for that of Hepatitis B e antigen (HBeAg) at 100 microg/ml after 9 d of treatment. The inhibitory activity of astragaloside IV on secretion of HBV antigens is more potent than that of 3TC without significant cytotoxicity. In duck hepatitis B virus (DHBV)-infected ducklings, astragaloside IV caused 64.0% inhibition at 120 mg/kg, 49.6% inhibition at 40 mg/kg, and 41.7% inhibition at 10 mg/kg to serum DHBVs after 10 d of treatment, and also reduced serum DHBV DNA levels. Together, our results demonstrate that astragaloside IV possesses potent anti-HBV activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astragalus Plant / chemistry*
  • Carcinoma
  • Cell Line, Tumor
  • Dose-Response Relationship, Drug
  • Drugs, Chinese Herbal / pharmacology*
  • Ducks
  • Hepatitis B Surface Antigens / metabolism
  • Hepatitis B Virus, Duck / drug effects
  • Hepatitis B Virus, Duck / genetics
  • Hepatitis B e Antigens / metabolism
  • Hepatitis B virus / drug effects*
  • Humans
  • Saponins / pharmacology*
  • Transfection / methods
  • Triterpenes / pharmacology*


  • Drugs, Chinese Herbal
  • Hepatitis B Surface Antigens
  • Hepatitis B e Antigens
  • Saponins
  • Triterpenes
  • astragaloside A