Analysis of the fluorescence temporal point-spread function in a turbid medium and its application to optical imaging

J Biomed Opt. Nov-Dec 2008;13(6):064038. doi: 10.1117/1.3042271.


A time-domain optical method to evaluate the concentration (n), lifetime (tau), and depth (d) of a fluorescent inclusion is described by the complete analysis of the fluorescence temporal point-spread function (TPSF). The behavior of parameters in the fluorescence TPSF is explored, and we demonstrate the method with experimental data from a localized fluorescent inclusion in scattering media to recover images of n, tau, and d. The method has potential application for in vivo fluorescence imaging.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Computer Simulation
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / methods*
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*
  • Models, Biological*
  • Models, Chemical
  • Nephelometry and Turbidimetry / methods*
  • Phantoms, Imaging
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / methods*