KCTD10 interacts with proliferating cell nuclear antigen and its down-regulation could inhibit cell proliferation

J Cell Biochem. 2009 Feb 15;106(3):409-13. doi: 10.1002/jcb.22026.


A novel gene (GenBank accession No. AF113208) named KCTD10 (potassium channel tetramerisation domain-containing 10) was cloned from our 5300 EST database of human aorta cDNA library. Computational analysis showed that KCTD10 cDNA is 2,638 bp long, encoding 313 amino acids with a proliferating cell nuclear antigen binding motif, mapped to chromosome 12q24.11 with 7 exons, ubiquitously expressed in all 12 tested normal tissues and 7 of 8 tested tumor cell lines from MTN membranes by Northern blot. Nuclear localization of KCTD10 was observed in A549 cells. Yeast two-hybrid analysis and immunoprecipitation assay showed that KCTD10 can interact with PCNA. In A549 cells, KCTD10 down-regulation could inhibit cell proliferation, but its over-expression could not influence cell proliferation. The results suggest that KCTD10 may be associated with DNA synthesis and cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Cell Line, Tumor
  • Cell Proliferation
  • Down-Regulation*
  • Humans
  • Organ Specificity
  • Potassium Channels, Voltage-Gated / genetics
  • Potassium Channels, Voltage-Gated / metabolism*
  • Proliferating Cell Nuclear Antigen / metabolism*
  • Protein Binding
  • RNA, Messenger / genetics


  • KCTD10 protein, human
  • Potassium Channels, Voltage-Gated
  • Proliferating Cell Nuclear Antigen
  • RNA, Messenger