RNA interference screening demystified

J Clin Pathol. 2009 Mar;62(3):195-200. doi: 10.1136/jcp.2008.058735. Epub 2009 Jan 6.


Genetic screens, where the effects of modifying gene function on cell behaviour are assessed in a systematic fashion, have for some time provided useful information to those interested in disease pathogenesis and treatment. Genetic screens exploiting the phenomenon of RNA interference (RNAi) are now becoming commonplace. This article explains the different RNAi screen formats and describes some of the applications of RNAi screening that may be pertinent to the research pathologist.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Drug Resistance, Neoplasm / genetics
  • Gene Library
  • Genetic Testing / methods*
  • Humans
  • Myelodysplastic Syndromes / genetics
  • RNA Interference*
  • RNA, Small Interfering / genetics
  • Virus Diseases / genetics


  • RNA, Small Interfering