[Knockdown and overexpression of miR-219 lead to embryonic defects in zebrafish development]

Fen Zi Xi Bao Sheng Wu Xue Bao. 2008 Oct;41(5):341-8.
[Article in Chinese]


MicroRNAs (miRNAs) are important post-transcriptional modulators of gene expression, which have been implicated in many physiological and pathological processes. In this study, we found that miR-219 expressed since early segmentation stages (16s) in spinal cord and mid-, hindbrain in zebrafish using Northern blotting and whole mounts ISH technologies. Moreover, knockdown or overexpression of miR-219 led to specific embryonic defects. Furthermore, TUNEL assay showed that overexpression of miR-219 induced significant cell apoptosis in the head and tail of zebrafish. Our study suggested that miR-219 may play an important role in zebrafish embryonic development.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Embryo, Nonmammalian / metabolism
  • Gene Expression Regulation, Developmental / genetics
  • In Situ Nick-End Labeling
  • MicroRNAs / genetics*
  • MicroRNAs / physiology*
  • Microinjections
  • Zebrafish / embryology*
  • Zebrafish / genetics*


  • MicroRNAs