Effect of Prolyl-hydroxyproline (Pro-Hyp), a food-derived collagen peptide in human blood, on growth of fibroblasts from mouse skin

J Agric Food Chem. 2009 Jan 28;57(2):444-9. doi: 10.1021/jf802785h.


We examined the effect of prolyl-hydroxyproline (Pro-Hyp), which occurs in human peripheral blood after ingestion of collagen peptide, on the migration and growth of mouse skin fibroblasts. Mouse skin discs were cultured on a 24-well plastic plate in a fetal bovine serum (FBS)-free medium. Addition of Pro-Hyp (200 nmol/mL) significantly increased the number of fibroblasts migrating from the skin to the plate after incubation for 72 h. This effect of Pro-Hyp was abolished by the addition of mitomycin C. The fibroblasts that had migrated from the mouse skin were collected and cultured on collagen gel. The growth of fibroblasts on the collagen gel was suppressed even in the presence of FBS, while rapid fibroblast growth was observed on the plastic plate. Addition of Pro-Hyp (0-1000 nmol/mL) to the medium containing 10% FBS enhanced the growth of fibroblasts on the collagen gel in a dose-dependent manner. These results suggest that Pro-Hyp might stimulate the growth of fibroblasts in the skin and consequently increase the number of fibroblasts migrating from the skin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Migration Assays
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Collagen / blood
  • Collagen / metabolism*
  • Dipeptides / blood
  • Dipeptides / pharmacology*
  • Fibroblasts / drug effects*
  • Fibroblasts / physiology
  • Growth Substances / blood
  • Growth Substances / pharmacology*
  • Humans
  • In Vitro Techniques
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Peptides / blood
  • Peptides / pharmacology*
  • Skin / drug effects*


  • Dipeptides
  • Growth Substances
  • Peptides
  • prolyl-4-hydroxyproline
  • Collagen