KIBRA Co-localizes with protein kinase Mzeta (PKMzeta) in the mouse hippocampus

Biosci Biotechnol Biochem. 2009 Jan;73(1):147-51. doi: 10.1271/bbb.80564. Epub 2009 Jan 7.


KIBRA is a WW domain-containing protein that can bind to protein kinase Czeta (PKCzeta). The SNP of the ninth intron of the KIBRA gene is associated with human episodic memory performance. Protein kinase Mzeta (PKMzeta), a brain-specific variant of PKCzeta, plays important roles in memory formation. Here we examined the interaction of KIBRA and PKMzeta in the adult mouse brain. Immunoprecipitation using newly-raised anti-KIBRA antibody revealed the interaction between KIBRA and PKMzeta in the brain. KIBRA was co-localized with PKMzeta in a single cultured neuron. Distribution analysis by immunohistochemistry and in situ hybridization indicated that KIBRA was highly localized with PKMzeta in the hippocampal CA1, CA3, and dentate gyrus. These results suggest that KIBRA functions in memory performance via interaction with PKMzeta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / metabolism*
  • Dentate Gyrus / chemistry
  • Hippocampus / chemistry*
  • Intracellular Signaling Peptides and Proteins
  • Memory*
  • Mice
  • Neurons / chemistry
  • Phosphoproteins
  • Protein Binding
  • Protein Kinase C / metabolism*


  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Phosphoproteins
  • Wwc1 protein, mouse
  • protein kinase C zeta
  • Protein Kinase C