MicroRNA-15b regulates cell cycle progression by targeting cyclins in glioma cells

Biochem Biophys Res Commun. 2009 Mar 6;380(2):205-10. doi: 10.1016/j.bbrc.2008.12.169. Epub 2009 Jan 9.

Abstract

MicroRNAs (miRNAs) are non-protein-coding RNAs that function as post-transcriptional gene regulators. Recent evidence has shown that miRNA plays a pivotal role in the development of many cancers including glioma, a lethal brain cancer. We have recently compared the miRNA expression profiles between normal brain and glioma tissues from Chinese patients by miRNA microarray and identified a panel of differentially expressed miRNAs. Here, we studied the function of one miRNA, miR-15b, in glioma carcinogenesis and elucidated its downstream targets. Over-expression of miR-15b resulted in cell cycle arrest at G0/G1 phase while suppression of miR-15b expression resulted in a decrease of cell populations in G0/G1 and a corresponding increase of cell populations in S phase. We further showed that CCNE1 (encoding cyclin E1) is one of the downstream targets of miR-15b. Taken together, our findings indicate that miR-15b regulates cell cycle progression in glioma cells by targeting cell cycle-related molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain / metabolism
  • Brain / pathology
  • Brain Neoplasms / genetics
  • Brain Neoplasms / pathology*
  • Cell Cycle / genetics*
  • Cell Line, Tumor
  • Cell Transformation, Neoplastic / genetics
  • Cell Transformation, Neoplastic / pathology
  • Cyclins / genetics*
  • Gene Expression Regulation, Neoplastic*
  • Glioma / genetics
  • Glioma / pathology*
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*

Substances

  • Cyclins
  • MIRN15 microRNA, human
  • MicroRNAs