Replisome stalling and stabilization at CGG repeats, which are responsible for chromosomal fragility

Nat Struct Mol Biol. 2009 Feb;16(2):226-8. doi: 10.1038/nsmb.1527. Epub 2009 Jan 11.

Abstract

Expanded CGG repeats cause chromosomal fragility and hereditary neurological disorders in humans. Replication forks stall at CGG repeats in a length-dependent manner in primate cells and in yeast. Saccharomyces cerevisiae proteins Tof1 and Mrc1 facilitate replication fork progression through CGG repeats. Remarkably, the fork-stabilizing role of Mrc1 does not involve its checkpoint function. Thus, chromosomal fragility might occur when forks stalled at expanded CGG repeats escape the S-phase checkpoint.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • COS Cells
  • Chlorocebus aethiops
  • Chromosome Fragility*
  • DNA Replication*
  • Humans
  • Saccharomyces cerevisiae / metabolism
  • Trinucleotide Repeats*