Large-scale profiling of protein palmitoylation in mammalian cells

Nat Methods. 2009 Feb;6(2):135-8. doi: 10.1038/nmeth.1293. Epub 2009 Jan 11.


S-palmitoylation is a pervasive post-translational modification required for the trafficking, compartmentalization and membrane tethering of many proteins. We demonstrate that the commercially available compound 17-octadecynoic acid (17-ODYA) can serve as a bioorthogonal, click chemistry probe for in situ labeling, identification and verification of palmitoylated proteins in human cells. We identified approximately 125 predicted palmitoylated proteins, including G proteins, receptors and a family of uncharacterized hydrolases whose plasma membrane localization depends on palmitoylation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fatty Acids, Unsaturated / analysis
  • Fatty Acids, Unsaturated / metabolism*
  • GTP-Binding Proteins / metabolism*
  • Humans
  • Jurkat Cells
  • Lipoylation
  • Protein Processing, Post-Translational*
  • Proteomics / methods*


  • Fatty Acids, Unsaturated
  • 17-octadecynoic acid
  • GTP-Binding Proteins