hShroom1 links a membrane bound protein to the actin cytoskeleton

Cell Mol Life Sci. 2009 Feb;66(4):681-96. doi: 10.1007/s00018-009-8645-1.


hShroom1 (hShrm1) is a member of the Apx/Shroom (Shrm) protein family and was identified from a yeast two-hybrid screen as a protein that interacts with the cytoplasmic domain of melanoma cell adhesion molecule (MCAM). The characteristic signature of the Shrm family is the presence of a unique domain, ASD2 (Apx/Shroom domain 2). mRNA analysis suggests that hShrm1 is expressed in brain, heart, skeletal muscle, colon, small intestine, kidney, placenta and lung tissue, as well a variety of melanoma and other cell lines. Co-immunoprecipitation and bioluminescence resonance energy transfer (BRET) experiments indicate that hShrm1 and MCAM interact in vivo and by immunofluorescence microscopy some co-localization of these proteins is observed. hShrm1 partly co-localises with beta-actin and is found in the Triton X-100 insoluble fraction of melanoma cell extracts. We propose that hShrm1 is involved in linking MCAM to the cytoskeleton.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism*
  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • CD146 Antigen / genetics
  • CD146 Antigen / metabolism
  • Cell Line
  • Cytoskeleton / metabolism*
  • Humans
  • Melanoma / genetics
  • Melanoma / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Microfilament Proteins / genetics
  • Microfilament Proteins / metabolism*
  • Molecular Sequence Data
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Two-Hybrid System Techniques


  • Actins
  • CD146 Antigen
  • MCAM protein, human
  • Membrane Proteins
  • Microfilament Proteins
  • Protein Isoforms
  • Recombinant Fusion Proteins
  • Shroom1 protein, human