Efficient processing of an antigenic sequence for presentation by MHC class I molecules depends on its neighboring residues in the protein

Cell. 1991 Sep 20;66(6):1145-53. doi: 10.1016/0092-8674(91)90037-y.

Abstract

Processing of endogenously synthesized proteins generates short peptides that are presented by MHC class I molecules to CD8 T lymphocytes. Here it is documented that not only the sequence of the presented peptide but also the residues by which it is flanked in the protein determine the efficiency of processing and presentation. This became evident when a viral sequence of proven antigenicity was inserted at different positions into an unrelated carrier protein. Not different peptides, but different amounts of the antigenic insert itself were retrieved by isolation of naturally processed peptides from cells expressing the different chimeric proteins. Low yield of antigenic peptide from an unfavorable integration site could be overcome by flanking the insert with oligo-alanine to space it from disruptive neighboring sequences. Notably, the degree of protection against lethal virus disease related directly to the amount of naturally processed antigenic peptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine / chemistry
  • Amino Acid Sequence
  • Animals
  • Antigen-Presenting Cells / immunology*
  • Antigens, Viral / chemistry*
  • Antigens, Viral / immunology
  • Cell Line
  • H-2 Antigens / physiology*
  • Hepatitis B Core Antigens / immunology*
  • In Vitro Techniques
  • Major Histocompatibility Complex
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Peptides / immunology*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / immunology
  • Structure-Activity Relationship
  • T-Lymphocytes / immunology

Substances

  • Antigens, Viral
  • H-2 Antigens
  • Hepatitis B Core Antigens
  • Peptides
  • Recombinant Proteins
  • Alanine