Induction of anti-tumour immunity by dendritic cells transduced with hTERT recombinant adenovirus in mice

J Pathol. 2009 Apr;217(5):685-92. doi: 10.1002/path.2493.


Dendritic cells (DCs) transfected with recombinant, replication-defective adenovirus (Ad) vectors encoding the human telomerase reverse transcriptase (hTERT) are potent inducers of cytotoxic T lymphocytes (CTLs) and anti-tumour immunity. However, previous studies have mostly been in vitro. In this study, we sought to determine whether DCs transfected with hTERT (DC/Ad-hTERT) could elicit a potent anti-tumour immunogenic response in vivo. We found that murine DCs transfected with recombinant adenovirus encoding the hTERT gene (DC/Ad-hTERT) induced hTERT-specific CTLs in vivo effectively, compared with Ad-LacZ-transduced DC (DC/Ad-LacZ) controls. These hTERT-specific CTLs lysed various tumour cell lines in an hTERT-specific and MHC-I molecule-restricted fashion. We also found that DC/Ad-hTERT could increase antigen-specific T-cell proliferation and augment the number of IFN-gamma secreting T-cells in mice. These data suggest that the DC/Ad-hTERT vaccine may induce anti-tumour immunity against tumour cells expressing hTERT in an MHC-I molecule-restricted fashion in vivo through the augmentation of the hTERT-specific CTL response. The DC/Ad-hTERT vaccine may thus be used as an efficient DC-based tumour vaccine in clinical applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Animals
  • Cancer Vaccines / immunology*
  • Cytotoxicity, Immunologic / immunology
  • Dendritic Cells / immunology*
  • Dendritic Cells / transplantation
  • Female
  • Genetic Vectors
  • HLA-A2 Antigen / metabolism
  • Immunophenotyping
  • Interferon-gamma / biosynthesis
  • Mice
  • Mice, Inbred C57BL
  • T-Lymphocytes, Cytotoxic / immunology
  • Telomerase / genetics
  • Telomerase / immunology*
  • Transduction, Genetic
  • Tumor Cells, Cultured


  • Cancer Vaccines
  • HLA-A2 Antigen
  • Interferon-gamma
  • TERT protein, human
  • Telomerase