Selective recognition of unnatural imidazopyridopyrimidine:naphthyridine base pairs consisting of four hydrogen bonds by the Klenow fragment

J Am Chem Soc. 2009 Feb 11;131(5):1644-5. doi: 10.1021/ja807391g.


In this work, we investigated how thermally stable ImO(N):NaN(O) and ImN(O):NaO(N) pairs are recognized by the Klenow fragment (KF). As a result, these complementary base pairs, especially the ImN(O):NaO(N) pair, were recognized selectively due to the four hydrogen bonds between the nucleobases and the shape complementarity of the Im:Na pair similar to the purine:pyrimidine base pair.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pairing
  • DNA Polymerase I / chemistry*
  • Hydrogen Bonding
  • Imidazoles / chemistry
  • Kinetics
  • Naphthyridines / chemistry*
  • Nucleotides / chemistry
  • Pyrimidines / chemistry*


  • Imidazoles
  • Naphthyridines
  • Nucleotides
  • Pyrimidines
  • DNA Polymerase I