Purpose: To evaluate the function of cbfalpha1 on BMP-2 signaling to extracellular matrix proteins in dental papilla cells in vitro.
Methods: RT-PCR and Western blot were performed to detect the expression of ALP, OC, ON, OPN, BSP, DMP-1 and DSPP in cultured dental papilla cells induced by 200ng/mL BMP-2 and/or down-regulated by cbfalpha1 antisense technology, the results were analysed with SPSS 11.0 software package.
Results: We found that the amount of ALP and OC and the expression of OPN, BSP and ON were upregulated significantly after the cells were treated with BMP-2. After transfected with antisense cbfalpha1, the cells downregulated the expression of ALP, OC, OPN and BSP significantly(P<0.01).
Conclusions: As a kind of transcription factor, cbfalpha1 could be an important tache in the BMP-2 signal networks controlling cells differentiation and mineralization.