Reverse calcium affinity purification of Fab with calcium derivatized hydroxyapatite

J Immunol Methods. 2009 Mar 15;342(1-2):115-8. doi: 10.1016/j.jim.2008.11.021. Epub 2009 Jan 14.


This study introduces the application of calcium-derivatized hydroxyapatite for purification of Fab. Fab binds to native hydroxyapatite but fails to bind to the calcium derivatized form. IgG, Fc, and most other protein contaminants bind to the calcium form. This supports Fab purification by a simple flow-through method that achieves greater than 95% purity from papain digests and mammalian cell culture supernatants. Alternatively, Fab can be concentrated on native hydroxyapatite then eluted selectively by conversion to the calcium-derivatized form.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Calcium / chemistry*
  • Chromatography, Affinity / methods*
  • Culture Media
  • Durapatite / chemistry*
  • Immunoglobulin Fab Fragments / isolation & purification*
  • Immunoglobulin G / isolation & purification


  • Culture Media
  • Immunoglobulin Fab Fragments
  • Immunoglobulin G
  • Durapatite
  • Calcium