Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei

Mol Biochem Parasitol. 2009 Apr;164(2):131-6. doi: 10.1016/j.molbiopara.2008.12.008. Epub 2008 Dec 27.


Synchronisation of the Trypanosoma brucei cell cycle proved elusive for many years. A recent report demonstrated that synchronisation of procyclic form cells was possible following treatment with hydroxyurea. Here, that work is extended to the disease-relevant, mammalian-infective bloodstream stage trypanosome. Treatment of bloodstream stage Lister 427 T. brucei cells growing in vitro with 10 microg ml(-1) hydroxyurea for 6h led to an enrichment of cells in S phase. Following removal of the drug, cells proceeded uniformly through one round of the cell cycle, providing a much needed tool to enrich for specific cell cycle stages, in a manner similar to hydroxyurea treatment of procyclic form T. brucei.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle / drug effects*
  • Growth Inhibitors / pharmacology*
  • Hydroxyurea / pharmacology*
  • S Phase / drug effects
  • Trypanosoma brucei brucei / drug effects*
  • Trypanosoma brucei brucei / growth & development*


  • Growth Inhibitors
  • Hydroxyurea