In Vivo Imaging of Extracellular Matrix Remodeling by Tumor-Associated Fibroblasts

Nat Methods. 2009 Feb;6(2):143-5. doi: 10.1038/nmeth.1295. Epub 2009 Jan 18.

Abstract

Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Movement / physiology
  • Collagen / metabolism*
  • Extracellular Matrix / metabolism
  • Extracellular Matrix / pathology*
  • Fibroblasts
  • Humans
  • Image Enhancement / methods
  • Integrin beta Chains / metabolism
  • Mice
  • Mice, Transgenic
  • Microscopy, Confocal / methods*
  • Relaxin / pharmacology
  • Soft Tissue Neoplasms / metabolism
  • Soft Tissue Neoplasms / pathology*
  • Stromal Cells / metabolism
  • Stromal Cells / pathology

Substances

  • Integrin beta Chains
  • Relaxin
  • Collagen