Large-scale Gene Disruption Using the UAU1 Cassette

Methods Mol Biol. 2009;499:175-94. doi: 10.1007/978-1-60327-151-6_17.

Abstract

Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Candida albicans / genetics*
  • DNA Transposable Elements
  • Gene Silencing*
  • Genetic Techniques*
  • Mutagenesis, Insertional / methods*

Substances

  • DNA Transposable Elements