Control of yeast GAL genes by MIG1 repressor: a transcriptional cascade in the glucose response

EMBO J. 1991 Nov;10(11):3373-7.

Abstract

Glucose repression is a global regulatory mechanism in yeast. We have investigated how glucose regulates the GAL genes, which are required for galactose fermentation. We found that the GAL genes are controlled by a transcriptional cascade. Thus, GAL4, which encodes an activator of the GAL genes, is repressed by MIG1, a zinc finger protein that binds to the GAL4 promoter. MIG1 has a dual role in control of the GAL genes, since MIG1 also binds to the promoter of GAL1, a gene regulated by GAL4. A disruption of MIG1 interacts synergistically with a disruption of GAL80, a gene involved in galactose induction. This suggests that the MIG1-dependent response to glucose is amplified by down-regulation of the induction pathway.

MeSH terms

  • Base Sequence
  • Blotting, Northern
  • DNA Fingerprinting
  • Down-Regulation
  • Galactose / genetics*
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal*
  • Genes, Suppressor*
  • Molecular Sequence Data
  • Plasmids
  • Promoter Regions, Genetic
  • RNA, Fungal / analysis
  • Saccharomyces cerevisiae / genetics*
  • Transcription, Genetic*
  • Zinc Fingers / drug effects*
  • Zinc Fingers / genetics

Substances

  • RNA, Fungal
  • Galactose