Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system

Eur J Immunol. 1991 Oct;21(10):2591-7. doi: 10.1002/eji.1830211041.


A mouse lymphocyte surface molecule which is selectively expressed by mucosal T cells and detected with the monoclonal antibody (mAb) M290 has provisionally been identified as a beta 7 integrin. Identification was based on close homology of its beta subunit at the N terminus with the recently reported, highly distinctive, human beta 7 sequence. mAb were prepared against the beta and alpha subunits of the mouse molecule, termed beta 7 and alpha M290, respectively, and used to study surface expression of the two components. beta 7 was present on most lymph node lymphocytes in association with alpha 4 rather than alpha M290. This integrin, beta 7 alpha 4, was shown to be identical to LPAM-1 (beta p alpha 4) the Peyer's patch homing receptor. Stimulation in vitro of mouse lymph node T cells with anti-CD3 in the presence of transforming growth factor (TGF)-beta increased beta 7 expression in about 40% of cells and changed the associated alpha chain from alpha 4 to the novel alpha M290 subunit, which, in most cells, was expressed de novo. Immunoprecipitation of beta 7 both from these cells and from intraepithelial lymphocytes gave closely similar results and showed predominance of the beta 7 alpha M290 integrin. It is suggested that in vivo this change in alpha-chain usage occurs in mucosal T cells in response to TGF-beta acting in the mucosal microenvironment and that the new integrin confers particular adhesive properties, possibly homing specificity, on the cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Blotting, Western
  • Flow Cytometry
  • Integrins / chemistry
  • Integrins / immunology*
  • Integrins / metabolism
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / immunology*
  • Lymph Nodes / cytology
  • Macromolecular Substances
  • Mice
  • Peyer's Patches / cytology
  • Peyer's Patches / immunology
  • Precipitin Tests
  • T-Lymphocytes / metabolism*
  • Transforming Growth Factor beta / pharmacology


  • Antibodies, Monoclonal
  • Integrins
  • Macromolecular Substances
  • Transforming Growth Factor beta