Identification of an S-antigen-like molecule in Drosophila melanogaster: an immunohistochemical study

Exp Eye Res. 1991 Aug;53(2):171-8. doi: 10.1016/0014-4835(91)90071-l.

Abstract

Vision is a highly complex process common to many vertebrate and invertebrate species. The visual system of Drosophila represents a valuable model in which to study this process since its chromosomal makeup has been well mapped and many mutants containing specific photoreceptor defects have been described. S-antigen is a well-characterized photoreceptor cell protein which plays a fundamental role in the visual process. In this study, we used a panel of four monoclonal antibodies (MAbs) specific for amino, mid and carboxy terminal amino acid sequences in S-antigen in order to examine S-antigen immunoreactivity in the Drosophila visual system. Two MAbs, MAb5C6.47 and MAbC10-C10, localized S-antigen to the retinules and ocelli. In contrast, MAbA2-G5 and MAbA9-C6 did not stain. The presence and strong antigenic similarity of an S-antigen homolog in Drosophila suggests that in both vertebrates and invertebrates S-antigen plays a major role in the regulation of the phototransduction of vision.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens / analysis*
  • Arrestin
  • Autoantigens / analysis
  • Blotting, Western
  • Drosophila melanogaster / immunology*
  • Eye Proteins / analysis*
  • Immunohistochemistry

Substances

  • Antigens
  • Arrestin
  • Autoantigens
  • Eye Proteins