Proteomic analysis of plasma membrane vesicles

Angew Chem Int Ed Engl. 2009;48(9):1656-9. doi: 10.1002/anie.200803898.

Abstract

A simple and scalable method is presented for harvesting, purification, and on-chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flowcell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by LC-MS/MS. Over 93% of the detected proteins are plasma-membrane-derived.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / chemistry*
  • Chromatography, Liquid
  • Membrane Proteins / analysis
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism
  • Microfluidics / methods
  • Peptides / analysis
  • Proteomics / methods*
  • Tandem Mass Spectrometry

Substances

  • Membrane Proteins
  • Peptides