Legionella pneumophila Dot/Icm translocated substrates: a sum of parts

Curr Opin Microbiol. 2009 Feb;12(1):67-73. doi: 10.1016/j.mib.2008.12.004. Epub 2009 Jan 20.


Legionella pneumophila is an intracellular pathogen of freshwater amoeba and of alveolar macrophages in human hosts. After phagocytosis, L. pneumophila establishes a unique intracellular vacuolar niche that avoids entry into the lysosomal network. Critical for L. pneumophila intracellular growth is the Dot/Icm type IVB translocation system. Although over 80 substrates of the Dot/Icm apparatus have been identified, individual substrates are often genetically redundant, complicating their analysis. Deletion of critical Dot/Icm translocation system components causes a variety of defects during intracellular growth. Many of these effects on the host cell likely result from the actions of one or more Dot/Icm translocated substrates. Loss of single substrates never generates the profound effects observed in strains lacking translocation system components.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amoeba / microbiology
  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Gene Deletion
  • Humans
  • Legionella pneumophila / pathogenicity*
  • Macrophages / microbiology
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism*
  • Virulence Factors / genetics
  • Virulence Factors / metabolism*


  • Bacterial Proteins
  • Membrane Transport Proteins
  • Virulence Factors