Human Zn-alpha 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues

FEBS Lett. 1991 Sep 23;290(1-2):247-9. doi: 10.1016/0014-5793(91)81271-9.

Abstract

Two cDNA clones coding for Zn-alpha 2-glycoprotein (Zn-alpha 2-gp) have been isolated from a human breast library and their nucleotide sequences determined. The deduced amino acid sequence contains the coding information for a hydrophobic signal peptide and the 278 residues of the mature protein. Comparison of this sequence with that from the protein purified from plasma reveals four differences: two amino acid changes (Gln-67 and Glu-222) and insertion of two residues (Ile-75 and Phe-76). Northern-blot analysis showed that the Zn-alpha 2-gp gene is expressed in liver and normal breast, but not in placenta, ovary and thyroid. A comparative analysis in mammary tissues from women with different diseases revealed enhanced expression of Zn-alpha 2-gp gene in benign breast lesions and a variable expression level in breast cancers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Breast Neoplasms / genetics*
  • Cloning, Molecular
  • DNA / genetics
  • Female
  • Gene Expression
  • Glycoproteins / genetics*
  • Humans
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • RNA, Messenger / genetics
  • Restriction Mapping
  • Seminal Plasma Proteins*

Substances

  • Glycoproteins
  • Oligonucleotides
  • RNA, Messenger
  • Seminal Plasma Proteins
  • Zn-alpha-2-glycoprotein
  • DNA

Associated data

  • GENBANK/S55977
  • GENBANK/S55979
  • GENBANK/S55982
  • GENBANK/S57642
  • GENBANK/X58288
  • GENBANK/X58289
  • GENBANK/X58801
  • GENBANK/X58802
  • GENBANK/X58803
  • GENBANK/X59766